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PCR FOR MTB

PCR FOR MTBPCR FOR MTB

PCR FOR MTB

Method of Collection:

Blood in EDTA VIAL, Urine for PCR - First Morning urine sample for 5 consecutive days Sputum for PCR - Sputum sample for 3 consecutive days


Days for reporting:

7 Days


Reference:

Interpretation :
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Result Comments
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Mycobacterium tuberculosis Infection likely with any of the following species M. tuberculosis,
complex- Detected M. bovis, M. microti and M. africanum

Non tuberculous Mycobacteria Infection likely with Mycobacterium other than tuberculosis
detected complex.

Inhibition detected Inhibitors detected in the sample provided. Repeat sample is
recommended.

Mycobacterium tuberculosis complex Mycobacteria not detected in the sample provided.
& non tuberculosis Mycobacteria- Not detected

Note
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1. This test includes 3 targets of which 2 are for Mycobacterium tuberculosis complex (IS6110 & MPB64) and 1 target for Mycobacterium genus (16sRNA)
2. Limit of detection of the assay is 1-10 Mycobacteria per PCR.
3. This test does not differentiate between the Mycobacteria species.
4. Mycobacterium culture is recommended in case inhibition is detected.

Comments
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Mycobacterium tuberculosis complex ( M. tuberculosis, M. bovis, M. microti & M. africanum) are the only Mycobacteria that are transmitted from person to person and therefore are of public health importance. Non tuberculous Mycobacteria most commonly encountered are M. avium complex & M. kansasii which causes Pulmonary disease; M. abscessus, M. chelonae, M. marinum & M. fortuitum which cause skin and soft tissue infections. Many of the non tuberculous Mycobacteria are environmental contaminants. Nucleic acid amplification tests provide direct detection of various Mycobacteria.

 


Why is the Test Done?


How to prepare for the Test:


2000/-